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Spatial heterogeneity of the T cell receptor repertoire

  1. e heterogeneity of the T cell antigen receptor (TCR) repertoire
  2. The assessment of spatial heterogeneity of lung perfusion with 13 NN-saline PET may serve as a vascular biomarker in COPD
  3. Spatial heterogeneity of lung aeration and strain (change volume/resting volume) occurs at microscopic levels and contributes to lung injury. Yet, it is mostly assessed with histograms or large regions-of-interest. Spatial heterogeneity could also influence regional gene expression. We used positron emission tomography (PET)/computed tomography.

We determined CV of four different regional lung volumes as an aggregate measure of ventilation heterogeneity because this measure was previously applied in studies aiming to quantify the spatial variation in ventilation distribution using other examination techniques (8, 43, 52) The substantial spatial and temporal heterogeneity of indexes of inflammation in our surfactant depletion model of lung injury, ventilated with large tidal volumes and low PEEP, has implications for design and interpretation of studies in similar models of lung injury Academic Radiology . Spatial Heterogeneity of Lung Strain and Aeration and Regional Inflammation During Early Lung Injury Assessed with PET/CT . Motta-Ribeiro, G;Winkler, T;Hashimoto, S;Vidal Melo, MF

Somatic mutations together with immunoediting drive extensive heterogeneity within non-small-cell lung cancer (NSCLC). Herein we examine heterogeneity of the T cell antigen receptor (TCR).. Spatial transcriptomics inferred from pathology whole-slide images links tumor heterogeneity to survival in breast and lung cancer Alona Levy-Jurgenson 1 , Xavier Tekpli 2 , 3 9017. Background: Temporal and spatial heterogeneity of PD-L1 has been reported. However, its impact on clinical benefit from immune checkpoint inhibitor (ICI) has not been clearly defined. Methods: We queried the MD Anderson Lung Cancer GEMINI database and compared PD-L1 expression (tumor proportion score by immunohistochemistry using FDA-approved antibodies) in NSCLC specimens from different. Within individual tumors, highly multiplexed protein spatial profiling revealed coexistence of immune desert, inflamed, and excluded regions, which matched histologic pattern composition. Our results provide a detailed molecular map of lung adenocarcinoma intratumor spatial heterogeneity, tracing nongenetic routes of cancer evolution

Spatial Heterogeneity of Lung Perfusion Assessed with 13N

  1. Our results provide a detailed molecular map of lung adenocarcinoma intratumor spatial heterogeneity, tracing nongenetic routes of cancer evolution. Significance: Lung adenocarcinomas are classified based on histologic pattern prevalence. However, individual tumors exhibit multiple patterns with unknown molecular features
  2. The model is compared to experimental data in normal lungs. We confirm that spatial heterogeneity in pulmonary perfusion is time-dependent, and predict key features of pulmonary hypertensive disease using a simple implementation of increased vascular stiffness
  3. Tracing the Innate Genetic Evolution and Spatial Heterogeneity in Treatment Naïve Lung Cancer Lesions. Jihye Kim. Translational Bioinformatics and Cancer Systems Biology Laboratory. Division of Medical Oncology, Dept. Medicine, School of Medicine, University of Colorado Anschutz Medical Campu
  4. demonstrate heterogeneous levels of SARS-CoV-2 RNA which correlate with duration of disease and show a range of host immune response patterns as well as significant spatial heterogeneity of both viral load and immune response. RESULTS Detection of SARS-CoV-2 in Human Lung Autopsy Tissue Associated with Duration of Diseas
  5. Lung adenocarcinoma (ADC) is the most prevalent subtype of lung cancer and characterized by considerable morphological and mutational heterogeneity. However, little is known about the epigenomic intratumor variability between spatially separated histological growth patterns of ADC

The lung TRACERx study provided us with a unique setting in which to systematically study genomic and immunological intratumoral heterogeneity in the context of NSCLC. We explored the spatial.. Non-small cell lung cancer is a tumor with extensive histological heterogeneity caused by spatial and temporal genomic changes, posing major challenges for the treatment and prognosis of patients with lung adenocarcinoma In this study, spatial genetic heterogeneity of lung adenocarcinoma was compared by analyzing differences in 16 genes with known actionable mutations between primary and metastatic L/N. NSCLC tissues with confirmed EGFR, KRAS, and ALK driver mutations were randomly selected from our institutional tissue archives. The studies from the East Asian. Overcoming acquired resistance against targeted therapies to improve outcomes of lung cancer patients harboring driver mutations is a critical issue. While drug therapy oriented to a resistance mechanism appears attractive, spatial heterogeneity of resistance mechanisms in each patient will diminish treatment efficacy

48 Our results provide a detailed molecular map of lung adenocarcinoma intra-tumor spatial 49 heterogeneity, tracing non-genetic routes of cancer evolution. 50 51 Statement of significance 52 Lung adenocarcinomas are classified based on histologic pattern prevalence. However, individua Spatial heterogeneity of radiolabeled choline positron emission tomography in tumors of patients with non-small cell lung cancer: first-in-patient evaluation of [18 F]fluoromethyl-(1,2-2 H 4)-choline

Spatial and temporal heterogeneity of regional lung

Cystic fibrosis (CF) is the most common fatal autosomal recessive disorder in white populations. Radiographic and immunological studies demonstrated the spatial heterogeneity of disease severity and clinical manifestations in the CF lung (1, 2).The apical lobes typically suffer the most extensive deterioration and inflammatory disease; the basal lobes exhibit more air trapping (1-3) (A) The intensity and spatial heterogeneity of the 18 F-FDG net uptake rate (K i) increased with time. (A, B) During the first 6 h of injury, K i increased significantly in all lung regions and heterogeneity of K i became more pronounced than at baseline

r heterogeneity in COPD patients is greater than in healthy subjects, mainly because of the contribution of large lung regions and not because of changes in tissue density or r. The assessment of spatial heterogeneity of lung perfusion with 13 NN-saline PET may serve as a vascular biomarker in COPD Spatial Heterogeneity of Lung Perfusion Assessed with 13N PET as a Vascular Biomarker in Chronic Obstructive Pulmonary Disease Marcos F. Vidal Melo1, Tilo Winkler1, R. Scott Harris2, Guido Musch1, Reginald E. Greene3, and Jose G. Venegas1 1Department of Anesthesia and Critical Care, Massachusetts General Hospital and Harvard Medical School, Boston,. demonstrate heterogeneous levels of SARS-CoV-2 RNA which correlate with duration of disease and show a range of host immune response patterns as well as significant spatial heterogeneity of both viral load and immune response. RESULTS Detection of SARS-CoV-2 in Human Lung Autopsy Tissue Associated with Duration of Diseas However, whether comparable spatial heterogeneity in microbiota is present in the lungs of healthy subjects is unknown. This study tested whether spatial variation exists within the lung microbiota of healthy subjects. We hypothesized that intrasubject variation, though present, would be less than variation between subjects Spatial and temporal heterogeneity of PD-L1 and its impact on benefit from immune checkpoint blockade in non-small cell lung cancer (NSCLC) J. Clin. Oncol. , 37 ( 2019 ) , 10.1200/JCO.2019.37.15_suppl.901

heterogeneous (present in at least one, but not all, regions). Spatial intratumor heterogeneity was identified in all seven NSCLCs, with a me-dian of 30% heterogeneous mutations (range 4 SCIENCE sciencemag.org 10 OCTOBER 2014• VOL 346 ISSUE 6206 251 1Cancer Research UK Lung Cancer Centre of Excellence Protective mechanical ventilation (MV) aims at limiting global lung deformation and has been associated with better clinical outcomes in acute respiratory distress syndrome (ARDS) patients. In ARDS lungs without MV support, the mechanisms and evolution of lung tissue deformation remain understudied. In this work, we quantify the progression and heterogeneity of regional strain in injured lungs. Applying our methods to breast and lung cancer slides, we show a significant statistical link between heterogeneity and survival. Our methods potentially open a new and accessible approach to investigating tumor heterogeneity and other spatial molecular properties and their link to clinical characteristics, including treatment susceptibility.

Deepening understanding in the heterogeneity of tumors is critical for clinical treatment. Here we investigate tissue-wide gene expression heterogeneity throughout a multifocal lung cancer using the spatial transcriptomics (ST) technology. We identified gene expression gradients in stroma adjacent to tumor regions that allow for re-understanding of the tumor micro-environment The relationship of SARS-CoV-2 lung infection and severity of pulmonary disease is not fully understood. We analyzed autopsy specimens from 24 patients who succumbed to SARS-CoV-2 infection using a combination of different RNA and protein analytical platforms to characterize inter- and intra-patient heterogeneity of pulmonary virus infection. There was a spectrum of high and low virus cases.

Spatial and temporal heterogeneity of ventilator

  1. Lung cancer is the most common cancer in China. Previous studies have indicated that lung cancer incidence exhibits remarkable spatial heterogeneity, and lung cancer is related to outdoor air pollution. However, the non-linear spatial association between outdoor air pollution and lung cancer incidence in China remains unclear. In this study, the relationships between the lung cancer incidence.
  2. Asthma is one of the leading causes of disease burden when measured in terms of disability adjusted life years, despite low prevalence of self-reported cases among young women. This paper deals with the meso-scale correlates and spatial heterogeneity in the prevalence of self-reported Asthma across 640 districts in India, using a nationally representative sample of 699,686 women aged 15-49.
  3. Intratumor heterogeneity (ITH) of genomic alterations may impact prognosis of lung adenocarcinoma (LUAD). Here, we investigate ITH of somatic copy number alterations (SCNAs), DNA methylation, and.
  4. ing whether BAL-sampled microbiota vary among patients with IPF with different radiographic features.
  5. In conclusion, using scRNA-seq data and spatial mapping, the large degree of heterogeneity of ECs was identified and dissected into a multi-lineage interaction in BLM-treated and control lungs. These data provided important insights into the lineage relationships, homeostatic, and pathologic roles of ECs

Spatial transcriptomics inferred from pathology whole

Spatial and temporal heterogeneity of PD-L1 and its impact

  1. Case Studies of the Spatial Heterogeneity of DNA Viruses in the Cystic Fibrosis Lung Abstract Send to Citation Mgr. Add to Favorites. Email to a Friend. Track Citations. Case Studies of the Spatial Heterogeneity of DNA Viruses in the Cystic Fibrosis Lung as well as by directly damaging lung tissue via carcinomas and herpesviral outbreaks
  2. antly subpleural and paraseptal Enlarged, remodeled airspaces with thickened walls; Usually filled with mucu
  3. Little is known of the geospatial architecture of individual cell populations in lung adenocarcinoma (LUAD) evolution. Here, we perform single-cell RNA sequencing of 186,916 cells from 5 early-stage LUADs and 14 multi-region normal lung tissues of defined spatial proximities from the tumors. We show that cellular lineages, states, and transcriptomic features geospatially evolve across normal.

the perceived or measured spatial variation in the gray levels; coarse textures have larger Imaging Heterogeneity in Lung Cancer: Techniques, Applications, and Challenges Usman Bashir1 Muhammad Musib Siddique 1 Emma Mclean2 Vicky Goh1,3 Gary J. Cook1,4 Bashir U, Siddique MM, Mclean E, Goh V, Cook GJ 1Department of Cancer Imaging, Division of. Immune checkpoint inhibitors (ICIs) represent one of the most promising therapeutic approaches in metastatic non-small cell lung cancer (M-NSCLC). Unfortunately, approximately 50-75% of patients do not respond to this treatment modality. Intratumor heterogeneity (ITH) at the genetic and phenotypic level is considered as a major cause of anticancer therapy failure, including resistance to ICIs

Spatial Tumor Heterogeneity in Lung Cancer with Acquired Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor Resistance. Matthias Scheffler. Carsten Kobe. Matthias Scheffler. Carsten Kobe. as no concomitant antineoplastic treatment was allowed, and crizotinib treatment was restarted. Three weeks later, again a nearly complete metabolic. However, spatial relationships between pixel values in both images, which add to their degree of heterogeneity, are captured in GLCMs and their derived values. For example, second-order entropy, which measures degree of heterogeneity is high for heterogenous tumor shown in A and low for homogenous tumor shown in B (3.15 and 2.37, respectively) The goals of the present study were to (a) define the dose-volume effect curves for radiation pneumonitis in mouse lung, (b) define the threshold volume, and (c) further investigate the spatial heterogeneity of the radiosensitivity of mouse lung. Methods and Materials: Eight fractional volumes ranging from 94% to 17% of the lungs of C3Hf/Kam. While the ventilation heterogeneity latter studies included the entire lung, the extent of both ventilation and blood flow heterogeneity within This study described a temporal variance of 1-9% a single transverse caudal slice is usually represen- of the spatial variance, where the spatial variance was tative of the heterogeneity measured for. Programmed-death ligand 1 protein was measured in 49 non-small-cell lung cancer cases with 2 validated PD-L1 antibodies (E1L3N [Cell Signaling Technology] and SP142 [Spring Bioscience]) using chromogenic immunohistochemistry (IHC) and quantitative immunofluorescence (QIF) to assess heterogeneity and determine assay concordance

Nongenetic Evolution Drives Lung Adenocarcinoma Spatial

The ability to evaluate geographic heterogeneity of cancer incidence and mortality is important in cancer surveillance. Many statistical methods for evaluating global clustering and local cluster patterns are developed and have been examined by many simulation studies. However, the performance of these methods on two extreme cases (global clustering evaluation and local anomaly (outlier. The results have been posted as a preprint, Temporal and Spatial Heterogeneity of Host Response to SARS-CoV-2 Pulmonary Infection, on the medRxiv preprint server Background: Studies have shown that extensive genetic or spatial heterogeneity is present within tumors.The present study explored the influence of heterogeneous cells in the tumor microenvironment (TME) on the sensitivity of EGFR-mutant lung adenocarcinoma cells to EGFR-TKIs and further investigated associated molecular mechanisms

FlashPath - Lung - Cryptogenic Organizing Pneumonia

Spatial heterogeneity. The lung environment is heterogeneous, with a variety of environmental attributes differing over regions of the lung. Both ventilation (the amount of air passed into a region of the lung during inhalation) and perfusion (the amount of blood supplied to a region of the lung for oxygen exchange) show regional variances. The registration fitting between the MRI proton mask and the mesh lung airway tree. The mesh lung is represented as tiny dots and can be distinguished from the MRI data which has the obvious slice acquisition method... 70 Figure 15 conditions, then this can explain heterogeneity but not the spatial clustering. What principle organises the spatial structure? This question has been partially answered by Anafi and Wilson (2001), and Venegas et al. (2005), who suggest that pressures felt by any individual airway in the lung are related to the inflation of nearby lung tissue

Temporal and Spatial Heterogeneity in Pulmonary Perfusion

  1. She was awarded a K01 Career Development Award (2017) from the National Heart, Lung, and Blood Institute, entitled Assessing the spatial heterogeneity in cardiovascular risk factors within and between blacks and whites. As the PI of this grant, Dr. Tabb has mapped and measured the varying disparities locally in major cities as well as.
  2. Non-genetic evolution drives lung adenocarcinoma spatial heterogeneity and progression Daniele Tavernari, Elena Battistello, Elie Dheilly, Aaron S. Petruzzella, Marco Mina, Jessica Sordet-Dessimoz
  3. - Mass spectrometry imaging analysis can visually plot the spatial distribution heterogeneity of EGFR mutation status, which may benefit our understanding of tumor heterogeneity. Introduction Lung cancer is a highly heterogeneous malignant epithelial tumor with distinct pathological features and clinical behavior ( 1 )
  4. Ma B, Bates JHT (2014) Mechanical interactions between adjacent airways in the lung. J Appl Physiol 116:628-634. Article Google Scholar Nekorkin VI, Makarov VA, Kazantsev VB, Velarde MG (1997) Spatial disorder and pattern formation in lattices of coupled bistable elements. Phys D Nonlinear Phenom 100(3):330-34
  5. Lung adenocarcinoma (LAC) is composed of lepidic, papillary, mucinous, micropapillary and solid components in its parenchyma. Complex responses to therapeutics result from intratumoral heterogeneity. However, it remains confused that what components in a mixed LAC tumor are responsible to the heterogeneous EGFR mutation and PD-L1 expression. We investigated EGFR status via laser.

http://www.theaudiopedia.com What is SPATIAL HETEROGENEITY? What does SPATIAL HETEROGENEITY mean? SPATIAL HETEROGENEITY meaning - SPATIAL HETEROGE.. Spatial and temporal dissection of the genomic changes occurring during the evolution of human non-small cell lung cancer (NSCLC) may help elucidate the basis for its dismal prognosis. We sequenced 25 spatially distinct regions from seven operable NSCLCs and found evidence of branched evolution, with driver mutations arising before and after subclonal diversification Joshi K, de Massy MR, Ismail M, Reading JL, Uddin I, Woolston A, et al. Spatial heterogeneity of the T cell receptor repertoire reflects the mutational landscape in lung cancer. Nat Med. 2019;25(10):1549-59 Spatial heterogeneity of nanomedicine investigated by multiscale imaging of the drug, the nanoparticle and the tumour environment . Josanne Sophia de Maar 1, Alexandros Marios Sofias 2, Tiffany Porta Siegel 3, Rob J. Vreeken 3,4, Chrit Moonen 1, Clemens Bos 1, Roel Deckers 1 . 1 Introduction: Data regarding the pre-treatment intertumor heterogeneity of potential biomarkers in advanced-stage lung cancers is limited. A finding of such heterogeneity between primary and metastatic lesions would prove valuable to determine if a metastatic lesion can be a surrogate for the primary tumor, as more biomarkers will likely be used in the future to inform treatment decisions

Global DNA methylation reflects spatial heterogeneity and

Spatial heterogeneity of the T cell receptor repertoire reflects the mutational landscape in lung cancer. Kroopa Joshi, Marc Robert de Massy, Mazlina Ismail, James L. Reading, Imran Uddin, Annemarie Woolston, Emine Hatipoglu, Theres Oakes, Rachel Rosenthal,. Spatial Heterogeneity Is a Fundamental Feature of the Tumor Microenvironment. It is important to recognize that the orchestrated influence of microenvironmental components on cancer is often accompanied by strong regional differences (Gillies et al. 2012; Junttila and de Sauvage 2013).Evidence of spatial variations has been well documented in pathological observations (Clemente et al. 1996. Lung-Fei Lee University Chaired Professor Lee.1777@osu.edu 614.292.5508. 475 Arps Hall 1945 N. High St. Columbus, OH 43210 Static models may capture both spatial correlation and spatial heterogeneity. Spatial dynamic panels study spatial and temporal economic processes. Possible spatial cointegraton of regional markets can be detected in a.

Genetic heterogeneity of actionable genes between primary

Spatial heterogeneity of acquired resistance mechanisms to

Lung cancer is the leading cause of cancer-related death worldwide,1,2 with non-small-cell lung cancer (NSCLC) being the most common type. CRUK0020, and CRUK0063), and spatial heterogeneity. Indeed, increased tumor heterogeneity on unenhanced CT or MR images has been associated with poor prognosis in patients with lung cancer (10-13). Although contrast material-enhanced studies allow visualization of more diverse intratumoral components, the optimal assessment time after contrast material injection ( 14 ) and the predictive.

Deep-learning-assisted biophysical imaging cytometry at

Measurement of spatial and antibody-based PD-L1 heterogeneity in non-small cell lung cancer. Patricia Gaule. x. Patricia Gaule. Search for articles by this author, Jamaal Rehman. x. Jamaal Rehman. Search for articles by this author, James William Smithy. x. James William Smithy. An increase of 19.5% (1 standard deviation) in FDG spatial uptake heterogeneity, is associated with a 43% increase in the risk of death. Conclusion. Quantification of the FDG spatial uptake heterogeneity of lung tumors has potential to add prognostic information to lung cancer staging beyond SUVmax and clinical stage information Together, our results reveal the marked heterogeneity of TAM populations in different tumor regions, with M2 TAM predominance, particularly at IM. Significance: This study underlines the significance of the density, spatial distribution, and gene expression of TAM phenotypes as prognostic factors for overall survival in lung cancer

The results of the genetic analyses clearly showed that the spatial and temporal genetic heterogeneity of the epidermal growth factor receptor gene status originated from an identical tumor ancestor. An alternative paradigm to determine a therapeutic strategy for a patient with lung cancer should be considered given the genetic heterogeneity. The aim of our study was to determine if EIT was able to assess spatial and temporal heterogeneity of ventilation during pulmonary function testing in 14 young (37 ± 10 yr, mean age ± SD) and 12 elderly (71 ± 9 yr) subjects without lung disease and in 33 patients with chronic obstructive pulmonary disease (71 ± 9 yr)

File:UIP Fibroblastic foci 1Tracking the Evolution of Non–Small-Cell Lung Cancer | NEJMVentilation (V ˙ A) per unit regional lung volume (ml min

Spatial heterogeneity of radiolabeled choline positron

We present an approach to capturing Spatial Heterogeneity, i.e., Habitat detection using texture information. First, we compute circular harmonic wavelets for small patches within a Region of Interest (RoI). Second, we cluster the patches in order to define the sub-regions of an image with similar texture patterns (habitats). Finally, information about the resulting clusters and their texture. Objective Endoscopic mucosal biopsies of primary gastric cancers (GCs) are used to guide diagnosis, biomarker testing and treatment. Spatial intratumoural heterogeneity (ITH) may influence biopsy-derived information. We aimed to study ITH of primary GCs and matched lymph node metastasis (LNmet). Design GC resection samples were annotated to identify primary tumour superficial (PTsup), primary.

The biggest hurdle to targeted cancer therapy is the inevitable emergence of drug resistance. Tumor cells employ different mechanisms to resist the targeting agent. Most commonly in EGFR-mutant non-small cell lung cancer, secondary resistance mutations on the target kinase domain emerge to diminish the binding affinity of first- and second-generation inhibitors Lung cancer still has a high mortality and morbidity. For further understanding the lung cancer, SCS is also been applied to lung cancer research to investigate its heterogeneity, metastasis, drug resistance, tumor microenvironment and many other issues. In this review, we summarized lung cancer research using SCS and their research achievements Spatial Heterogeneity and Branched Evolution in NSCLC. Previous efforts to characterise the cancer genome of NSCLC have involved the analysis of copy number alterations targeted sequencing of candidate cancer genes , and next-generation sequencing of genomes and/or exomes -. By interrogating the mutational spectrum of tumours, these. Spatial heterogeneity is important in understanding the landscape functions and spatial ecological processes. Spatial heterogeneity can be measured in terms of any resources, including soil structure, plant diversity, biomass, animal distributions, etc. Spatial heterogeneity is an early indicator of regime shifts The study published in Nature Communications titled, Temporal and Spatial Heterogeneity of Host Response to SARS-CoV-2 Pulmonary Infection, revealed the spatial heterogeneity of patients.

Case Studies of the Spatial Heterogeneity of DNA Viruses

Cough, sarcoidosis and idiopathic pulmonary fibrosis: raw

Lung Metabolic Activation as an Early Biomarker of Acute

EGFR as a Pharmacological Target in EGFR-Mutant Non-SmallImmuno-OncologySputum tumor DNA detection in lung cancer | Download Table